STEM CELL PICTURES

SIGMA shRNA RhoA and some controls

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Protocol

H9 hESCs grown in mTeSR1 on matrigel

Passaged 24hrs prior using Accutase

Transduced with virus twice

Lenti-shRNA treated d0=10ul, d1=30 uL of virus in 24 well dish (500 uL)

At d5 1ug/mL puromycin was added

At d9 present images were recorded

Groups

RhoA shRNA 08 (4x, 10x)

RhoA_shRNA_08_4x #1

RhoA_shRNA_08_4x #2

RhoA_shRNA_08_10x

RhoA shRNA 09 (4x, 10x)

RhoA_shRNA_09_4x #1

RhoA_shRNA_09_4x #2

RhoA_shRNA_09_10x

RhoA shRNA 10 (4x, 10x)

RhoA_shRNA_10_4x #1

RhoA_shRNA_10_4x #2

RhoA_shRNA_10_10x

RhoA shRNA 11 (4x, 10x) *may have recieved double virus d1

RhoA_shRNA_11_4x #1

RhoA_shRNA_11_4x #2

RhoA_shRNA_11_10x

RhoA shRNA 12 (4x, 10x)

RhoA_shRNA_12_4x #1

RhoA_shRNA_12_4x #2

RhoA_shRNA_12_10x

No shRNA with pgk-puro Control

scv0001control_4x #1

scv0001control_4x #2

scv0001control_10x

Scrambled shRNA with pgk-puro Control

scv0002control_4x #1

scv0002control_4x #2

scv0002control_10x

Lentivirus from lentigen - No pgk-puro and Non puro treated

lentigen_control_4x #1

lentigen_control_4x #2

lentigen_control_10x

Non-transfected with puro treatment

Non-Transduced with 1 ug/mL puro for 4d 4x #1

Non-Transduced with 1 ug/mL puro for 4d 4x #2

Non-Transduced with 1 ug/mL puro for 4d 10x

 


Please note, these photographs are property of Rutgers University, and Rick Cohen, Ph.D., and may not be used without expressed written permission.

W.M. Keck Center for Collaborative Neuroscience